Present study assessed with hemisphere specialization, emotional intelligence was found to differ. The influence of hemisphere specialization on emotional intelligence is the central research issue in discussion .In the present investigation, higher emotional intelligence is found in individuals with ambidextrous hemisphere specialization than individuals with right or left hemisphere specialization. Descriptive survey method was used for the present study. This study also explored the impact of hemisphere specialization on emotional intelligence based on gender. The findings have been discussed in the light of available theoretical and empirical literature.